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elisa plate reader  (BMG Labtech)
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BMG Labtech elisa plate reader
Elisa Plate Reader, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clariostar elisa plate reader  (BMG Labtech)
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BMG Labtech clariostar elisa plate reader
CD8 + T cells from individuals with hoFH validate a role of LDLR signaling for CD8 + T cell function (A) A schematic overview of the LDLR protein domains and LDLR-adaptor protein 1 (LDLRAP1), highlighting the hoFH mutations included in the experiments with blue reversed triangles. The empty reverse triangle shapes represent hoFH patients with mutations in the LDLR, while the filled reverse triangle shape represents hoFH patients with mutations in the LDLRAP1. (B) Cell surface LDLR gMFI levels on CD8 + T cells from hoFH patients ( n = 5) and healthy controls (HCs) ( n = 5). Cells were activated for the indicated duration of time with anti-CD3/CD28 Dynabeads and a cytokine mix (interleukin [IL]-2, IL-7, and IL-15). (C) Graphical illustration of the LDL-pHrodo uptake experiment. (D) Uptake of LDL-pHrodo measured by flow cytometry, where CD8 + T cells were activated for 24 h, followed by a 2-h culturing in lipoprotein-deprived medium and 2-h incubation with the LDL-pHrodo complex (20 μg/mL). Where indicated, anti-LDLR (5 μg/mL) was added when cells were cultured in lipoprotein-deprived medium. One-way ANOVA with Šídák’s multiple comparisons test, where ∗∗∗∗ p < 0.0001 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (E–H) Cells were activated for 72 h with anti-CD3/CD28 Dynabeads and cytokine mix (IL-2, IL-7, and IL-15). (E) Intracellular Ki67 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (F) Cell surface ICAM-1 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (G) Intracellular granzyme B levels measured with flow cytometry. Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). (H) Secreted granzyme B levels measured with <t>ELISA.</t> Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians.
Clariostar Elisa Plate Reader, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elisa+plate+reader/pmc12936826-321-5-9?v=BMG+Labtech
Average 98 stars, based on 1 article reviews
clariostar elisa plate reader - by Bioz Stars, 2026-07
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sunrise elisa plate reader  (Tecan Systems)
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Tecan Systems sunrise elisa plate reader
CD8 + T cells from individuals with hoFH validate a role of LDLR signaling for CD8 + T cell function (A) A schematic overview of the LDLR protein domains and LDLR-adaptor protein 1 (LDLRAP1), highlighting the hoFH mutations included in the experiments with blue reversed triangles. The empty reverse triangle shapes represent hoFH patients with mutations in the LDLR, while the filled reverse triangle shape represents hoFH patients with mutations in the LDLRAP1. (B) Cell surface LDLR gMFI levels on CD8 + T cells from hoFH patients ( n = 5) and healthy controls (HCs) ( n = 5). Cells were activated for the indicated duration of time with anti-CD3/CD28 Dynabeads and a cytokine mix (interleukin [IL]-2, IL-7, and IL-15). (C) Graphical illustration of the LDL-pHrodo uptake experiment. (D) Uptake of LDL-pHrodo measured by flow cytometry, where CD8 + T cells were activated for 24 h, followed by a 2-h culturing in lipoprotein-deprived medium and 2-h incubation with the LDL-pHrodo complex (20 μg/mL). Where indicated, anti-LDLR (5 μg/mL) was added when cells were cultured in lipoprotein-deprived medium. One-way ANOVA with Šídák’s multiple comparisons test, where ∗∗∗∗ p < 0.0001 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (E–H) Cells were activated for 72 h with anti-CD3/CD28 Dynabeads and cytokine mix (IL-2, IL-7, and IL-15). (E) Intracellular Ki67 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (F) Cell surface ICAM-1 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (G) Intracellular granzyme B levels measured with flow cytometry. Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). (H) Secreted granzyme B levels measured with <t>ELISA.</t> Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians.
Sunrise Elisa Plate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elisa+plate+reader/pm41855006-103-7-11?v=Tecan+Systems
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sunrise elisa plate reader - by Bioz Stars, 2026-07
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accureader automatic elisa plate reader  (Metertech Inc)
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Metertech Inc accureader automatic elisa plate reader
CD8 + T cells from individuals with hoFH validate a role of LDLR signaling for CD8 + T cell function (A) A schematic overview of the LDLR protein domains and LDLR-adaptor protein 1 (LDLRAP1), highlighting the hoFH mutations included in the experiments with blue reversed triangles. The empty reverse triangle shapes represent hoFH patients with mutations in the LDLR, while the filled reverse triangle shape represents hoFH patients with mutations in the LDLRAP1. (B) Cell surface LDLR gMFI levels on CD8 + T cells from hoFH patients ( n = 5) and healthy controls (HCs) ( n = 5). Cells were activated for the indicated duration of time with anti-CD3/CD28 Dynabeads and a cytokine mix (interleukin [IL]-2, IL-7, and IL-15). (C) Graphical illustration of the LDL-pHrodo uptake experiment. (D) Uptake of LDL-pHrodo measured by flow cytometry, where CD8 + T cells were activated for 24 h, followed by a 2-h culturing in lipoprotein-deprived medium and 2-h incubation with the LDL-pHrodo complex (20 μg/mL). Where indicated, anti-LDLR (5 μg/mL) was added when cells were cultured in lipoprotein-deprived medium. One-way ANOVA with Šídák’s multiple comparisons test, where ∗∗∗∗ p < 0.0001 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (E–H) Cells were activated for 72 h with anti-CD3/CD28 Dynabeads and cytokine mix (IL-2, IL-7, and IL-15). (E) Intracellular Ki67 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (F) Cell surface ICAM-1 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (G) Intracellular granzyme B levels measured with flow cytometry. Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). (H) Secreted granzyme B levels measured with <t>ELISA.</t> Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians.
Accureader Automatic Elisa Plate Reader, supplied by Metertech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elisa+plate+reader/pm41823867-65-11-16?v=Metertech+Inc
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accureader automatic elisa plate reader - by Bioz Stars, 2026-07
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elisa plate reader  (Tecan Systems)
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Tecan Systems elisa plate reader
CD8 + T cells from individuals with hoFH validate a role of LDLR signaling for CD8 + T cell function (A) A schematic overview of the LDLR protein domains and LDLR-adaptor protein 1 (LDLRAP1), highlighting the hoFH mutations included in the experiments with blue reversed triangles. The empty reverse triangle shapes represent hoFH patients with mutations in the LDLR, while the filled reverse triangle shape represents hoFH patients with mutations in the LDLRAP1. (B) Cell surface LDLR gMFI levels on CD8 + T cells from hoFH patients ( n = 5) and healthy controls (HCs) ( n = 5). Cells were activated for the indicated duration of time with anti-CD3/CD28 Dynabeads and a cytokine mix (interleukin [IL]-2, IL-7, and IL-15). (C) Graphical illustration of the LDL-pHrodo uptake experiment. (D) Uptake of LDL-pHrodo measured by flow cytometry, where CD8 + T cells were activated for 24 h, followed by a 2-h culturing in lipoprotein-deprived medium and 2-h incubation with the LDL-pHrodo complex (20 μg/mL). Where indicated, anti-LDLR (5 μg/mL) was added when cells were cultured in lipoprotein-deprived medium. One-way ANOVA with Šídák’s multiple comparisons test, where ∗∗∗∗ p < 0.0001 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (E–H) Cells were activated for 72 h with anti-CD3/CD28 Dynabeads and cytokine mix (IL-2, IL-7, and IL-15). (E) Intracellular Ki67 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (F) Cell surface ICAM-1 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (G) Intracellular granzyme B levels measured with flow cytometry. Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). (H) Secreted granzyme B levels measured with <t>ELISA.</t> Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians.
Elisa Plate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elisa+plate+reader/10__1016_slash_j__xcrp__2026__103101-288-17-21?v=Tecan+Systems
Average 99 stars, based on 1 article reviews
elisa plate reader - by Bioz Stars, 2026-07
99/100 stars
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CD8 + T cells from individuals with hoFH validate a role of LDLR signaling for CD8 + T cell function (A) A schematic overview of the LDLR protein domains and LDLR-adaptor protein 1 (LDLRAP1), highlighting the hoFH mutations included in the experiments with blue reversed triangles. The empty reverse triangle shapes represent hoFH patients with mutations in the LDLR, while the filled reverse triangle shape represents hoFH patients with mutations in the LDLRAP1. (B) Cell surface LDLR gMFI levels on CD8 + T cells from hoFH patients ( n = 5) and healthy controls (HCs) ( n = 5). Cells were activated for the indicated duration of time with anti-CD3/CD28 Dynabeads and a cytokine mix (interleukin [IL]-2, IL-7, and IL-15). (C) Graphical illustration of the LDL-pHrodo uptake experiment. (D) Uptake of LDL-pHrodo measured by flow cytometry, where CD8 + T cells were activated for 24 h, followed by a 2-h culturing in lipoprotein-deprived medium and 2-h incubation with the LDL-pHrodo complex (20 μg/mL). Where indicated, anti-LDLR (5 μg/mL) was added when cells were cultured in lipoprotein-deprived medium. One-way ANOVA with Šídák’s multiple comparisons test, where ∗∗∗∗ p < 0.0001 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (E–H) Cells were activated for 72 h with anti-CD3/CD28 Dynabeads and cytokine mix (IL-2, IL-7, and IL-15). (E) Intracellular Ki67 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (F) Cell surface ICAM-1 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (G) Intracellular granzyme B levels measured with flow cytometry. Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). (H) Secreted granzyme B levels measured with ELISA. Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians.

Journal: iScience

Article Title: PCSK9-mediated degradation of cell-surface LDL receptors impairs human CD8+ T cell effector functions

doi: 10.1016/j.isci.2026.114859

Figure Lengend Snippet: CD8 + T cells from individuals with hoFH validate a role of LDLR signaling for CD8 + T cell function (A) A schematic overview of the LDLR protein domains and LDLR-adaptor protein 1 (LDLRAP1), highlighting the hoFH mutations included in the experiments with blue reversed triangles. The empty reverse triangle shapes represent hoFH patients with mutations in the LDLR, while the filled reverse triangle shape represents hoFH patients with mutations in the LDLRAP1. (B) Cell surface LDLR gMFI levels on CD8 + T cells from hoFH patients ( n = 5) and healthy controls (HCs) ( n = 5). Cells were activated for the indicated duration of time with anti-CD3/CD28 Dynabeads and a cytokine mix (interleukin [IL]-2, IL-7, and IL-15). (C) Graphical illustration of the LDL-pHrodo uptake experiment. (D) Uptake of LDL-pHrodo measured by flow cytometry, where CD8 + T cells were activated for 24 h, followed by a 2-h culturing in lipoprotein-deprived medium and 2-h incubation with the LDL-pHrodo complex (20 μg/mL). Where indicated, anti-LDLR (5 μg/mL) was added when cells were cultured in lipoprotein-deprived medium. One-way ANOVA with Šídák’s multiple comparisons test, where ∗∗∗∗ p < 0.0001 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (E–H) Cells were activated for 72 h with anti-CD3/CD28 Dynabeads and cytokine mix (IL-2, IL-7, and IL-15). (E) Intracellular Ki67 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (F) Cell surface ICAM-1 levels measured with flow cytometry. Two-tailed Mann-Whitney test, where ∗ p < 0.05 ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians. (G) Intracellular granzyme B levels measured with flow cytometry. Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). (H) Secreted granzyme B levels measured with ELISA. Two-tailed Mann-Whitney test ( n = 5 HCs and n = 5 hoFH patients). Each dot represents data from a separate donor, and lines depict medians.

Article Snippet: Calorimetric measurements were performed using CLARIOstar ELISA plate reader (BMG Labtech).

Techniques: Cell Function Assay, Flow Cytometry, Incubation, Cell Culture, Two Tailed Test, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay